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400 ul ice-cold flow cytometry staining buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 400 ul ice-cold flow cytometry staining buffer
    A, Schema for splenocyte adoptive transfer experiments. Splenocytes were isolated from CD45.2 sham and HF mice 8 weeks after coronary ligation or sham operation and transferred to syngeneic CD45.1 mice. Recipient mice were then followed for an 8-week period. B, M-mode ECGs from recipient mice 8 weeks after receiving splenocytes from sham or HF mice. C, Serial group echocardiographic data for end-diastolic volume (EDV), end-systolic volume (ESV), and ejection fraction (EF) during the 8-week follow-up period after cell transfer (n=7 per group). D, Similar echocardiographic data in parallel groups of mice after adoptive transfer of splenocytes derived from donors treated with either lipopolysaccharide (LPS) or PBS control (n=7 per group). E, Representative hearts from mice receiving HF or sham splenocytes and corresponding heart and lung gravimetric group data. F, Flow <t>cytometry</t> scatter plots and quantification of circulating CD11b+F4/80+ monocytes in recipient mice 8 weeks after cell transfer. G, Examples of spleens harvested from mice receiving HF or sham splenocytes, corresponding spleen gravimetry, and representative trichrome-stained histological sections from the same. N=4 to 6 per group (E–G).
    400 Ul Ice Cold Flow Cytometry Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/400 ul ice-cold flow cytometry staining buffer/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    400 ul ice-cold flow cytometry staining buffer - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Remodeling of the Mononuclear Phagocyte Network Underlies Chronic Inflammation and Disease Progression in Heart Failure"

    Article Title: Remodeling of the Mononuclear Phagocyte Network Underlies Chronic Inflammation and Disease Progression in Heart Failure

    Journal: Circulation research

    doi: 10.1161/CIRCRESAHA.113.301720

    A, Schema for splenocyte adoptive transfer experiments. Splenocytes were isolated from CD45.2 sham and HF mice 8 weeks after coronary ligation or sham operation and transferred to syngeneic CD45.1 mice. Recipient mice were then followed for an 8-week period. B, M-mode ECGs from recipient mice 8 weeks after receiving splenocytes from sham or HF mice. C, Serial group echocardiographic data for end-diastolic volume (EDV), end-systolic volume (ESV), and ejection fraction (EF) during the 8-week follow-up period after cell transfer (n=7 per group). D, Similar echocardiographic data in parallel groups of mice after adoptive transfer of splenocytes derived from donors treated with either lipopolysaccharide (LPS) or PBS control (n=7 per group). E, Representative hearts from mice receiving HF or sham splenocytes and corresponding heart and lung gravimetric group data. F, Flow cytometry scatter plots and quantification of circulating CD11b+F4/80+ monocytes in recipient mice 8 weeks after cell transfer. G, Examples of spleens harvested from mice receiving HF or sham splenocytes, corresponding spleen gravimetry, and representative trichrome-stained histological sections from the same. N=4 to 6 per group (E–G).
    Figure Legend Snippet: A, Schema for splenocyte adoptive transfer experiments. Splenocytes were isolated from CD45.2 sham and HF mice 8 weeks after coronary ligation or sham operation and transferred to syngeneic CD45.1 mice. Recipient mice were then followed for an 8-week period. B, M-mode ECGs from recipient mice 8 weeks after receiving splenocytes from sham or HF mice. C, Serial group echocardiographic data for end-diastolic volume (EDV), end-systolic volume (ESV), and ejection fraction (EF) during the 8-week follow-up period after cell transfer (n=7 per group). D, Similar echocardiographic data in parallel groups of mice after adoptive transfer of splenocytes derived from donors treated with either lipopolysaccharide (LPS) or PBS control (n=7 per group). E, Representative hearts from mice receiving HF or sham splenocytes and corresponding heart and lung gravimetric group data. F, Flow cytometry scatter plots and quantification of circulating CD11b+F4/80+ monocytes in recipient mice 8 weeks after cell transfer. G, Examples of spleens harvested from mice receiving HF or sham splenocytes, corresponding spleen gravimetry, and representative trichrome-stained histological sections from the same. N=4 to 6 per group (E–G).

    Techniques Used: Adoptive Transfer Assay, Isolation, Ligation, Derivative Assay, Flow Cytometry, Staining



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    400 ul ice-cold flow cytometry staining buffer  (Thermo Fisher)
    90
    Thermo Fisher 400 ul ice-cold flow cytometry staining buffer
    A, Schema for splenocyte adoptive transfer experiments. Splenocytes were isolated from CD45.2 sham and HF mice 8 weeks after coronary ligation or sham operation and transferred to syngeneic CD45.1 mice. Recipient mice were then followed for an 8-week period. B, M-mode ECGs from recipient mice 8 weeks after receiving splenocytes from sham or HF mice. C, Serial group echocardiographic data for end-diastolic volume (EDV), end-systolic volume (ESV), and ejection fraction (EF) during the 8-week follow-up period after cell transfer (n=7 per group). D, Similar echocardiographic data in parallel groups of mice after adoptive transfer of splenocytes derived from donors treated with either lipopolysaccharide (LPS) or PBS control (n=7 per group). E, Representative hearts from mice receiving HF or sham splenocytes and corresponding heart and lung gravimetric group data. F, Flow <t>cytometry</t> scatter plots and quantification of circulating CD11b+F4/80+ monocytes in recipient mice 8 weeks after cell transfer. G, Examples of spleens harvested from mice receiving HF or sham splenocytes, corresponding spleen gravimetry, and representative trichrome-stained histological sections from the same. N=4 to 6 per group (E–G).
    400 Ul Ice Cold Flow Cytometry Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/400 ul ice-cold flow cytometry staining buffer/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    400 ul ice-cold flow cytometry staining buffer - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A, Schema for splenocyte adoptive transfer experiments. Splenocytes were isolated from CD45.2 sham and HF mice 8 weeks after coronary ligation or sham operation and transferred to syngeneic CD45.1 mice. Recipient mice were then followed for an 8-week period. B, M-mode ECGs from recipient mice 8 weeks after receiving splenocytes from sham or HF mice. C, Serial group echocardiographic data for end-diastolic volume (EDV), end-systolic volume (ESV), and ejection fraction (EF) during the 8-week follow-up period after cell transfer (n=7 per group). D, Similar echocardiographic data in parallel groups of mice after adoptive transfer of splenocytes derived from donors treated with either lipopolysaccharide (LPS) or PBS control (n=7 per group). E, Representative hearts from mice receiving HF or sham splenocytes and corresponding heart and lung gravimetric group data. F, Flow cytometry scatter plots and quantification of circulating CD11b+F4/80+ monocytes in recipient mice 8 weeks after cell transfer. G, Examples of spleens harvested from mice receiving HF or sham splenocytes, corresponding spleen gravimetry, and representative trichrome-stained histological sections from the same. N=4 to 6 per group (E–G).

    Journal: Circulation research

    Article Title: Remodeling of the Mononuclear Phagocyte Network Underlies Chronic Inflammation and Disease Progression in Heart Failure

    doi: 10.1161/CIRCRESAHA.113.301720

    Figure Lengend Snippet: A, Schema for splenocyte adoptive transfer experiments. Splenocytes were isolated from CD45.2 sham and HF mice 8 weeks after coronary ligation or sham operation and transferred to syngeneic CD45.1 mice. Recipient mice were then followed for an 8-week period. B, M-mode ECGs from recipient mice 8 weeks after receiving splenocytes from sham or HF mice. C, Serial group echocardiographic data for end-diastolic volume (EDV), end-systolic volume (ESV), and ejection fraction (EF) during the 8-week follow-up period after cell transfer (n=7 per group). D, Similar echocardiographic data in parallel groups of mice after adoptive transfer of splenocytes derived from donors treated with either lipopolysaccharide (LPS) or PBS control (n=7 per group). E, Representative hearts from mice receiving HF or sham splenocytes and corresponding heart and lung gravimetric group data. F, Flow cytometry scatter plots and quantification of circulating CD11b+F4/80+ monocytes in recipient mice 8 weeks after cell transfer. G, Examples of spleens harvested from mice receiving HF or sham splenocytes, corresponding spleen gravimetry, and representative trichrome-stained histological sections from the same. N=4 to 6 per group (E–G).

    Article Snippet: Leukocytes were collected by centrifugation (380 g for 10 minutes at 4°C) and resuspended in 400 uL ice-cold flow cytometry staining buffer (eBiosciences).

    Techniques: Adoptive Transfer Assay, Isolation, Ligation, Derivative Assay, Flow Cytometry, Staining